Document Type

Poster Presentation

Publication Date

4-24-2026

Year of Award

2026

Date Assignment Submitted

2026

Abstract

Widespread use of pesticides such as 2,4-dichlorophenoxyacetic acid (2,4-D) raises serious concerns about their effects on non-target aquatic organisms. In vertebrates, 2,4-D has been associated with neurotoxic outcomes such as impaired motor function and altered neurotransmitter activity. Planarians offer a valuable system for toxicological studies due to their well-characterized nervous system, regenerative capacity, and measurable behavioral responses. This study investigates the neurotoxic effects of 2,4-D exposure in the planarian species, Girardia dorotocephala, including assessment of nociceptive behaviors, survival rate, and phototactic responses. Worms were exposed to various concentrations of 2,4-D (0–100 mg/L) over multiple time points. Nociceptive responses such as scrunching, C-shapes, and seizures, survival, and mortality were monitored. Photophobic behavior was tested using wavelength-specific light stimuli to determine whether 2,4-D disrupts sensory-motor processing. Higher concentrations of 2,4-D induced abnormal nociceptive behaviors and increased mortality. However, phototaxis assays indicate 2,4-D does not impair phototaxis in planarians. These findings will contribute to a broader understanding of how environmental toxicants affect aquatic invertebrates at both behavioral and molecular levels and reinforce using planarians as a sensitive model for neurotoxicity and ecotoxicology research.

Publisher

Lynn University

Conference/Symposium

Lynn University Student Research Symposium

Contest

Poster Presentations: Natural Science category

City/State

Boca Raton, FL

Department

College of Arts and Sciences

Instructor

Faculty Sponsor: Dr. Cassandra S. Korte

Planaria_swimming_around_red_light.mov (1107 kB)
Planaria swimming around red light

Planaria_swimming_around_ultraviolet_light.mov (2143 kB)
Planaria swimming around ultraviolet light

Planaria_swimming_away_green_light.mov (1647 kB)
Planaria swimming away green light

Planaria_swimming_around_ultraviolet_light (2).mov (1472 kB)
Planaria swimming around ultraviolet light

Planaria_swimming_through_green_light.mov (1246 kB)
Planaria swimming through green light

Planaria_swimming_through_red_light.mov (4687 kB)
Planaria swimming through red light

Planaria_swimming_through_ultraviolet_light.mov (2892 kB)
Planaria swimming through ultraviolet light

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