Immunodetection and Quantification of Airborne (1-3)-β-D-glucan-carrying Particles with the Halogen Immunoassay

Document Type

Article

Publication Date

2-28-2013

DOI

https://doi.org/10.1016/j.jim.2012.11.009

Abstract

Fungal cell wall components, such as (1-3)-β-D-glucan, are known to be capable of activating the innate immune system and pose a respiratory health risk in different environments. Mass-based non-viable techniques commonly used for assessment of fungal exposures could be β-D-glucan-specific, but are limited to analysis of liquid extracts. The variable solubility of different β-D-glucans may underestimate β-D-glucan exposure and long sampling times required for mass-based methods make assessing short-term exposures difficult. In this study, we evaluated the utility of the halogen immunoassay (HIA), an immunoblotting technique previously used for allergens, to immunodetect and quantify β-D-glucan-carrying particles (BGCPs). The HIA was able to detect BGCPs without background staining when β-D-glucan standards and air samples collected at a poultry house during short sampling periods were evaluated. The image analysis protocol previously developed by our group for mouse allergen allowed simultaneous immunodetection and quantification of β-D-glucan-containing particles. Our results suggest that the HIA holds promise for quantifying β-D-glucan exposures. To our knowledge, this is the first time in which the HIA was used for non-allergenic compounds of microbial or fungal origins. © 2012 Elsevier B.V..

Publication

Journal of Immunological Methods

Volume

388

Issue

1-2

Department

College of Arts and Sciences

Peer Reviewed

1

Publication History

Received 4 September 2012, Revised 12 November 2012, Accepted 12 November 2012, Available online 29 November 2012.

Comments

Copyright © 2012 Elsevier B.V. All rights reserved.

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