Document Type

Oral Presentation

Publication Date

Spring 4-19-2024

Date Assignment Submitted



Traumatic brain injuries (TBI) are associated with immediate and long-term consequences that can be divided into primary and secondary injuries. A primary injury results from the direct damage inflicted on the brain by the impact, whereas a secondary injury takes time to manifest due to the cascade of molecular events that lead to cellular damage and death within the brain. This study analyzes mortality as a secondary injury following repetitive traumatic brain injuries in the presence of a human neurodegenerative genetic risk factor, APOE Îμ4. The High Impact Trauma (HIT) device developed by Katzenberger et al. (2013) was utilized to inflict TBI in Drosophila melanogaster animal models. First, the mortality index at 24 hours (MI24) was collected in a wild-type (w1118) fruit fly model subjected to varying numbers of TBIs. The data indicates a direct correlation between the number of TBIs inflicted and mortality. As the number of TBIs increases, the mortality index increases. This data is representative of what Katzenberger et al. (2013) demonstrated, indicating the efficacy of the HIT device. Using a genetic over-expression system, the amount of APOE Îμ4 was elevated in the fruit flies’ brains. Fly groups were subjected to either one or five TBIs. The mortality index was calculated at 24 hours, three days, and seven days post-TBI. The final results, along with analysis, will be presented during the presentation at the Symposium.


Lynn University


Lynn University Student Research Symposium


Oral Presentation


Boca Raton, FL


College of Arts and Sciences


Dr. Kimberly D. Rowland

Ashworth_Mortality of Fruit Flies, Following Traumatic Brain Injury (TBI).pptx (17116 kB)
Mortality of Fruit Flies, Following Traumatic Brain Injury (TBI)



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